Mixer Mill MM 400

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Mixer Mill MM 400

Mixer Mill MM 400 with quick release clamp
(please order grinding jars and balls separately)

20.715.0001

MM 400

100–240 V, 50/60 Hz

Grinding jars MM 400, screw top design

Selection help

Screw-top grinding jars are dust- and air-tight which makes them suitable for dry, wet and cryogenic grinding. Moreover, they safely enclose inert atmosphere, for example after the jar was filled inside a glove box.

Always select a grinding jar size suitable for the sample volume (approx. 30% of the jar is filled with sample material). Grinding jars are provided in sizes ranging from 1,5 ml to 50 ml (not all sizes available in all materials).

The document “Grinding Jar Ball Charge” in the Downloads section provides details about usable volumes, maximum feed sizes and correct filling of the jars with grinding balls for dry and wet milling as well as cell disruption.

For efficient and contamination-free grinding processes the grinding jars are available in 6 different materials (no undesired abrasion).

The energy input increases with the density of a material. For pulverizing soft samples, materials with a low to medium density should be used, whereas for hard samples it is better to use high-density materials.

Available materials:

Hardened steel (density 7.8 g/cm³; hardness ≤ 235 HB, medium abrasion resistance)
typically used for pulverizing medium-hard to hard and brittle samples.
Stainless steel (density 7.7 g/cm³; hardness ≤ 245 HB, medium abrasion resistance)
typically used for pulverizing medium-hard to hard and brittle samples.
Tungsten carbide (density 14.8 g/cm³; hardness 93.6 HRA; high abrasion resistance)
used for grinding very hard, brittle sample materials. By using tungsten carbide, contamination of the sample with iron is prevented.
Agate (density 2.6 g/cm³; hardness 6.5 – 7 Mohs, low abrasion resistance)
a highly pure natural material suitable for pulverizing soft to medium-hard or fibrous samples. Due to its low-density agate is suitable for gentle grinding processes with low energy input.
Zirconium oxide (density 6.05 g/cm³; hardness 1250 HV, high abrasion resistance)
ceramic material suitable for pulverizing medium-hard to hard and fibrous samples. It is typically used for heavy-metal-free grinding processes or applications which aim at ultrafine particle sizes.

Hardened steel

01.462.0237 25 ml

Stainless steel

01.462.0230 1.5 ml

01.462.0231 5 ml

01.462.0290 5 ml (for use with adapter 02.706.0351)

01.462.0236 10 ml

01.462.0213 25 ml

01.462.0214 35 ml

01.462.0216 50 ml

Tungsten carbide

01.462.0235 10 ml

01.462.0217 25 ml

Agate

01.462.0232 5 ml

01.462.0233 10 ml

Zirconium oxide

01.462.0234 10 ml

01.462.0201 25 ml

01.462.0215 35 ml

PTFE

01.462.0238 25 ml

01.462.0244 35 ml

05.068.0004 Mixing beakers of polystyrene, 28 ml, 100 pieces

05.068.0006 Mixing beakers of polystyrene, 56 ml, 100 pieces

PMMA, transparent jars for mechanosyntheses

01.462.0539 10 ml, 10 pieces

02.462.0539 10 ml, 2 pieces

Accessories for grinding jars MM 400

02.486.0005 Jar wrench for grinding jars

02.706.0351 Adapter for use of 2/4 grinding jars 5 ml (01.462.0290)

03.111.0229 Gasket for grinding jar 1.5 ml, 10 pieces

03.111.0231 Gasket for grinding jar 5 ml, 10 pieces (for grinding jar 01.462.0231)

03.111.0262 Gasket for grinding jar 5 ml, 10 pieces (for grinding jar 01.462.0290)

03.111.0232 Gasket for grinding jar 10 ml, 10 pieces

03.111.0228 Gasket for grinding jar 25 ml hardened steel and stainless steel, 10 pieces

03.111.0212 Gasket for grinding jar 25 ml zirconium oxide and tungsten carbide, 10 pieces

03.111.0224 Gasket for grinding jar 35 ml stainless steel, 10 pieces

03.111.0222 Gasket for grinding jar 35 ml zirconium oxide, 10 pieces

03.111.0209 Gasket for grinding jar 50 ml stainless steel, 10 pieces

Accessories for mixing and cell disruption MM 400

The conical centrifuge tubes are perfectly suitable for mixing powders or liquids, e. g. for the extraction of pesticide residues with the QuEChERS method.

They can also be used for enzyme extraction from cell suspensions – up to 8 x 30 ml cell suspension are disrupted in one step. Soft tissue like liver may also be homogenized in these tubes.

For biological applications like cell disruption or homogenization of small tissue samples a variety of single-use vials are available. For cell disruption glass beads are the best choice.

The document “Grinding Jar Ball Charge” in the Downloads section provides details about usable volumes, maximum feed sizes and correct filling of the jars with grinding balls for dry and wet milling as well as cell disruption.

For further questions please contact our application experts.

22.001.0020 Adapter for 4 conical centrifuge tubes (e.g. Falcon® Tubes), 2 pieces, incl. 20 tubes

05.026.0001 Conical centrifuge tubes, 50 ml, 20 pieces

22.001.0021 Adapter for 4 wide mouth bottles, 2 pieces, incl. 12 wide mouth bottles, 30 ml

Accessories for cold grinding MM 400

For cold grinding (cryogenic grinding) we recommend using the Cryo kit which consists of two pairs of tongs, two insulated containers (1 & 6 liters) as well as a pair of safety goggles.

Please only use grinding jars of hardened or stainless steel for cryogenic grinding. When using jars made of other materials, inner tension may build up and lead to damages of the jar under extreme temperature variations.

Attention:

First, the jar is filled with sample and ball(s), then the lid is screwed tightly, and the jar is immersed with the tongs in the container filled with liquid nitrogen. After a few minutes (the liquid nitrogen should no longer boil) the sample has been indirectly embrittled inside the jar which can now be removed with the help of the tongs and clamped into the mill. Please never fill liquid nitrogen into the grinding jar because it evaporates and causes a considerable overpressure inside the jar.

22.354.0001 Cryo kit for cooling the grinding jars with liquid nitrogen (incl. 2 insulated containers (1 and 4 liter), 2 pairs of grinding jar tongs, 1 pair of safety glasses)

Accessories MM 400

99.200.0043 IQ/OQ Documentation for MM 400

Accessories for cell and tissue disruption

For biological applications like cell disruption or homogenization of small tissue samples a variety of single-use vials are available. For cell disruption glass beads are the best choice.

The document “Grinding Jar Ball Charge” in the Downloads section provides details about usable volumes, maximum feed sizes and correct filling of the jars with grinding balls for dry and wet milling as well as cell disruption.

22.008.0010 Adapter for 5 reaction vials 5.0 ml, made of PTFE

22.008.0014 Adapter for 10 reaction vials 1.5 and 2.0 ml, made of PTFE or stainless steel

22.008.0005 Adapter for 5 reaction vials 1.5 and 2.0 ml, made of PTFE or stainless steel

22.008.0006 Adapter for 10 reaction vials 0.2 ml, made of PTFE

22.749.0006 Safe-lock reaction vials 5.0 ml, 200 pcs.

22.749.0001 Safe-lock reaction vials 2.0 ml, 1000 pcs.

22.749.0002 Safe-lock reaction vials 1.5 ml, 1000 pcs.

22.749.0004 Safe-lock reaction vials 0.2 ml, 1000 pcs.

22.749.0008 Reaction vials made of stainless steel 316L, 2.0 ml, 10 pcs.

Grinding balls

Selection help

Grinding balls and grinding jars should always be selected from the same material.

Grinding balls are available in different diameters. The rule of thumb is: for efficient milling the ball should be at least 3 times larger than the largest particle in the sample. To avoid abrasion and wear it is essential to only use balls with identical diameter.

The number of grinding balls depends on the jar volume used. The document “Grinding Jar Ball Charge” in the Downloads section provides details about usable volumes, maximum feed sizes and correct filling of the jars with grinding balls for dry and wet milling as well as cell disruption. For further information on materials please see the document “Material Analyses of Equipment & Accessories“ in the Downloads section.

Available materials:

Hardened steel (density 7.8 g/cm³; hardness ≤ 235 HB, medium abrasion resistance)
typically used for pulverizing medium-hard to hard and brittle samples.
Stainless steel (density 7.7 g/cm³; hardness ≤ 245 HB, medium abrasion resistance)
typically used for pulverizing medium-hard to hard and brittle samples.
Tungsten carbide (density 14.8 g/cm³; hardness 93.6 HRA; high abrasion resistance)
used for grinding very hard, brittle sample materials. By using tungsten carbide, contamination of the sample with iron is prevented.
Agate (density 2.6 g/cm³; hardness 6.5 – 7 Mohs, low abrasion resistance)
a highly pure natural material suitable for pulverizing soft to medium-hard or fibrous samples. Due to its low-density agate is suitable for gentle grinding processes with low energy input.
Zirconium oxide (density 6.05 g/cm³; hardness 1250 HV, high abrasion resistance) ceramic material suitable for pulverizing medium-hard to hard and fibrous samples. It is typically used for heavy-metal-free grinding processes or applications which aim at ultrafine particle sizes.

Glass beads are suitable for applications like cell disruption, for example of yeast, bacteria or microalgae. For yeast larger glass beads are best suited whereas microalgae require small beads. Glass beads are also used for washing bacteria off surfaces or tissue samples for subsequent diagnosis.